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Structured Review

Proteintech β tublin
β Tublin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2501 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B2+tublin/beta+Tubulin+Antibody/pm41803900-115-81-86
Average 96 stars, based on 2501 article reviews
β tublin - by Bioz Stars, 2026-10
96/100 stars

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Related Articles

Incubation:

Article Title: Microglia-specific interleukin-4 delivery by engineered extracellular vesicles restores inner blood-retinal barrier in diabetic retinopathy via GAS6-MERTK pathway
Article Snippet: .. The membranes were incubated with primary antibodies overnight at 4◦C against iNOS (1:1000; 22226-1-AP, Proteintech), TNF-α (1:1000; ab183218, Abcam), Arg-1 (1:5000; 16001-1-AP, Proteintech), CD206 (1 μg/mL; AF2535, R-D Systems) (1:1000; 24595S, Cell Signaling Technology), MERTK (1:500; DF7344, Affinity Biosciences), Phosopho-MERTK (pY753/685) (1:500; AF8443, Affinity Bioscience), and β-Tublin (1:20000; 66240-1-Ig, Proteintech) which was used as an internal reference. ..

Article Title: Microglia-specific interleukin-4 delivery by engineered extracellular vesicles restores inner blood-retinal barrier in diabetic retinopathy via GAS6-MERTK pathway.
Article Snippet: .. The membranes were incubated with primary antibodies overnight at 4◦C against iNOS (1:1000; 22226-1-AP, Proteintech), TNF-α (1:1000; ab183218, Abcam), Arg-1 (1:5000; 16001-1-AP, Proteintech), CD206 (1 μg/mL; AF2535, R-D Systems) (1:1000; 24595S, Cell Signaling Technology), MERTK (1:500; DF7344, Affinity Biosciences), Phosopho-MERTK (pY753/685) (1:500; AF8443, Affinity Bioscience), and β-Tublin (1:20000; 66240-1-Ig, Proteintech) which was used as an internal reference. ..

Article Title: Royal jelly protein hydrolysates accelerate in vitro diabetic wound healing by enhancing keratinocytes functions and suppressing macrophage-mediated inflammation.
Article Snippet: The FuturePAGETM gel (10%, ACE, China) was used for protein separation and the protein was transferred onto polyvinylidene fluoride (PVDF) membranes (0.45μm, Millipore, USA). .. The membranes were incubated with primary antibodies anti-INOS (1 : 1000, 18985-1-AP, proteintech, China), COX-2 (1 : 1000, 66351-1-Ig, proteintech, China), IKKα (1 : 1000, bs-2907R, Bioss, China), phospho-IKKα (p-IKKα) (1 : 1000, AP0505, Abclonal, China), IκBα (1 : 1000, 10268-1-AP, proteintech, China), phospho-IκBα (p-IκBα) (1 : 1000, 82349-1-RR, proteintech, China), p65 (1 : 1000, 380172, ZENBIO, China), phospho-p65 (pAR TIC LE IN PR ES S p65) (1 : 1000, 82335-1-RR, proteintech, China), β-Actin (1 : 10000, 66009-1-Ig, proteintech, China) and β-Tublin (1 : 5000, 10094-1-AP, proteintech, China) at 4°C overnight after blocked with Fish Gelatin Fast Blocking Kit (ZENBIO, China) and then incubated with secondary antibodies HRPconjugated Affinipure Goat Anti-Rabbit IgG(H+L) (1:20000, SA00001-2, proteintech, China) or HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) (1:20000, SA00001-1, proteintech, China)for 45 min at room temperature. .. Total RNA was extracted using an Eastep Super Total RNA Extraction Kit (LS1040, Shanghai Promega, China) AR TIC LE IN PR ES S and reverse-transcribed using the FastKing gDNA Dispelling RT SuperMix (TIANGEN, China).

SDS Page:

Article Title: FASN-mediated metabolic reprogramming drives CD4 + T cell hyperactivation in Sjögren’s syndrome via fatty acid oxidation-dependent oxidative phosphorylation
Article Snippet: Then protein extraction was carried out with RIPA lysis buffer (Beyotime, Shanghai, China). .. After SDS-PAGE separation and PVDF membrane transfer, the membrane was briefly sealed and detected overnight at 4 °C with primary antibodies targeting cell cycle regulators, including CDK2 (2546, CST, USA) and CDK4 (12790, CST, USA), oxidative phosphorylation related proteins, including COXIV (ab16056, Abcam, UK), ATP5A (ab176569, Abcam, UK), NDUFB8 (ab192878, Abcam, UK), FASN (3180, CST, USA) and β-Tublin (HRP-66240, Proteintech, China) (all diluted 1:1000). ..

Membrane:

Article Title: FASN-mediated metabolic reprogramming drives CD4 + T cell hyperactivation in Sjögren’s syndrome via fatty acid oxidation-dependent oxidative phosphorylation
Article Snippet: Then protein extraction was carried out with RIPA lysis buffer (Beyotime, Shanghai, China). .. After SDS-PAGE separation and PVDF membrane transfer, the membrane was briefly sealed and detected overnight at 4 °C with primary antibodies targeting cell cycle regulators, including CDK2 (2546, CST, USA) and CDK4 (12790, CST, USA), oxidative phosphorylation related proteins, including COXIV (ab16056, Abcam, UK), ATP5A (ab176569, Abcam, UK), NDUFB8 (ab192878, Abcam, UK), FASN (3180, CST, USA) and β-Tublin (HRP-66240, Proteintech, China) (all diluted 1:1000). ..

Phospho-proteomics:

Article Title: FASN-mediated metabolic reprogramming drives CD4 + T cell hyperactivation in Sjögren’s syndrome via fatty acid oxidation-dependent oxidative phosphorylation
Article Snippet: Then protein extraction was carried out with RIPA lysis buffer (Beyotime, Shanghai, China). .. After SDS-PAGE separation and PVDF membrane transfer, the membrane was briefly sealed and detected overnight at 4 °C with primary antibodies targeting cell cycle regulators, including CDK2 (2546, CST, USA) and CDK4 (12790, CST, USA), oxidative phosphorylation related proteins, including COXIV (ab16056, Abcam, UK), ATP5A (ab176569, Abcam, UK), NDUFB8 (ab192878, Abcam, UK), FASN (3180, CST, USA) and β-Tublin (HRP-66240, Proteintech, China) (all diluted 1:1000). ..

Blocking Assay:

Article Title: Royal jelly protein hydrolysates accelerate in vitro diabetic wound healing by enhancing keratinocytes functions and suppressing macrophage-mediated inflammation.
Article Snippet: The FuturePAGETM gel (10%, ACE, China) was used for protein separation and the protein was transferred onto polyvinylidene fluoride (PVDF) membranes (0.45μm, Millipore, USA). .. The membranes were incubated with primary antibodies anti-INOS (1 : 1000, 18985-1-AP, proteintech, China), COX-2 (1 : 1000, 66351-1-Ig, proteintech, China), IKKα (1 : 1000, bs-2907R, Bioss, China), phospho-IKKα (p-IKKα) (1 : 1000, AP0505, Abclonal, China), IκBα (1 : 1000, 10268-1-AP, proteintech, China), phospho-IκBα (p-IκBα) (1 : 1000, 82349-1-RR, proteintech, China), p65 (1 : 1000, 380172, ZENBIO, China), phospho-p65 (pAR TIC LE IN PR ES S p65) (1 : 1000, 82335-1-RR, proteintech, China), β-Actin (1 : 10000, 66009-1-Ig, proteintech, China) and β-Tublin (1 : 5000, 10094-1-AP, proteintech, China) at 4°C overnight after blocked with Fish Gelatin Fast Blocking Kit (ZENBIO, China) and then incubated with secondary antibodies HRPconjugated Affinipure Goat Anti-Rabbit IgG(H+L) (1:20000, SA00001-2, proteintech, China) or HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) (1:20000, SA00001-1, proteintech, China)for 45 min at room temperature. .. Total RNA was extracted using an Eastep Super Total RNA Extraction Kit (LS1040, Shanghai Promega, China) AR TIC LE IN PR ES S and reverse-transcribed using the FastKing gDNA Dispelling RT SuperMix (TIANGEN, China).



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